ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (1): 165-171.doi: 10.11843/j.issn.0366-6964.2016.01.022

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The Investigation of Infection and Multilocus Sequence of Giardia duodenalis in Dairy Cattle from an Imported Farm

WANG Chen-rong,ZHANG Zhen-jie,LI Jun-qiang,YU Fu-chang,CAO Jian-ke,ZHANG Long-xian*   

  1. (College of Animal Science and Veterinary Medicine,Henan Agricultural University,International Joint Research Laboratory for Zoonotic Diseases of Henan Province,Zhengzhou 450002,China)
  • Received:2015-06-20 Online:2016-01-23 Published:2016-01-23

Abstract:

We investigated the infection of Giardia duodenalis in dairy cattle from an imported farm,and then we analyzed the distribution of the genotypes and assemblage of G.duodenalis.The detection of G.duodenalis in dairy cattle base on the locus of SSU rRNA,tpigdh and bg by PCR. Results indicated that the prevalence of G.duodenalis in dairy cattle is 9.8% (48/507) based on SSU rRNA by PCR,and 48 positive samples were identified assemblage E.The prevalence of G.duodenalis in post-weaning calves was the highest (20.70%),the difference of the prevalence of G.duodenalis in cattle in different ages was significant.The 48 positive samples were amplified 19,28 and 34 base on tpigdh and bg locus respectively,one sample was mixed infection (assemblage A and assemblage E).Multilocus sequence and phylogenetic analysis indicated that G.duodenalis infecting dairy cattle from an imported farm may be from the local area. The results indicate that imported adult dairy cattle is not ease to carry Zoonotic G.duodenalis,dairy cattle from an imported farm have a lower risk to transmite Zoonotic G.duodenalis.

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